Elution Behavior of Carbohydrates for Core-Shell Ion-Exchange Resin St-50 with Different Degrees of Cross-Linking in the Porous Shell

Reducing the retention time in carbohydrate analysis is crucial to development of resins for application high-performance liquid chromatography (HPLC). In order solve this problem a new core-shell ion-exchange resin with thin shell layer consisting an ion-exchanging porous on hard polymer core was prepared use HPLC. The effect degree cross-linking (10–55%) shell, wherein core/shell monomer weight ratio 50:50 (St-50), and theoretical plate number (N) separated carbohydrates examined. A mixture inositol, glucose, fructose, sucrose under alkaline conditions (0.10 mol/L NaOH) at flow rates 0.3–0.7 mL/min using St-50, which exhibited 40 55%. found be related layer. values N were dependent all when 0.10 NaOH eluent used.